Try our NEW Urine Protein Concentration Calculator
Up to 20 ml starting volume
Concentrate up to 400x
Vivaspin 20 centrifugal devices allow rapid concentration of protein and nanoparticle samples up to 20 ml in volume. They can be used in rotors accepting 50 ml centrifuge tubes or used with air pressure. The patented twin vertical PES membrane design minimizes filter fouling while providing the fastest spin times. The built in dead stop pocket provides maximum concentrate recovery. Some Vivaspin 20 devices are available for in vitro diagnostic (IVD) applications and have been used to concentrate urine samples prior to electrophoresis for diagnosis of multiple myeloma, light chain amyloidosis and related blood disorders. Sebia and Helena Labs both recommend the use of Vivaspin 20 devices to prepare urine samples for their capillary electrophoresis systems.
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Time to concentrate up to 30x [min.] at 20°C and solute recovery % |
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| Mode | Centrifuge | Centrifuge | Benchtop | Pressure Fuge | ||||
| Type | Swing Bucket | 25° Fixed Angle | Pressure | Swing Bucket | ||||
| Pressure/Speed | 3,000 g | 6,000 g | 4 bar | 3,000 g + 4 bar | ||||
| Start volume | 20 ml | 14 ml | 10 ml | 10 ml | ||||
| Min. | Rec. | Min. | Rec. | Min. | Rec. | Min. | Rec. | |
| Cytochrome c 0.25 mg/ml (12,400 MW) | ||||||||
| 3,000 MWCO PES | 110 | 97% | 180 | 96% | 60 | 96% | - | - |
| BSA 1.0 mg / ml (66,000 MW) | ||||||||
| 5,000 MWCO PES | 23 | 99% | 29 | 99% | 50 | 98% | 14 | 98% |
| 10,000 MWCO PES | 16 | 98% | 17 | 98% | 32 | 97% | 8 | 97% | 30,000 MWCO PES | 13 | 98% | 15 | 98% | 32 | 97% | 8 | 97% |
| IgG 0.25 mg / ml (160,000 MW) | ||||||||
| 30,000 MWCO PES | 27 | 97% | 20 | 95% | 46 | 94% | 13 | 97% |
| 50,000 MWCO PES | 27 | 97% | 22 | 95% | 46 | 93% | 13 | 96% |
| 100,000 MWCO PES | 25 | 91% | 20 | 90% | 42 | 88% | 12 | 94% |
| Latex beads 0.004 % in DMEM +10 % FCS (0.055 µm) | ||||||||
| 300,000 MWCO PES | 20 | 99% | 35 | 99% | 10 | 99% | - | - |
| Latex beads 0.004 % in DMEM +10 % FCS (0.24 µm) | ||||||||
| 1,000,000 MWCO PES | 4 | 99% | 12 | 99% | 4 | 99% | - | - |
| Yeast 1.0 mg / ml (S. Cerevisiae) | ||||||||
| 0.2µm PES | 15 | 95% | 5 | 95% | 20 | 95% | 2 | 95% |
| Concentrator Capacity | |
| Swing Bucket Rotor | 20 ml |
| 25° Fixed Angle Rotor | 14 ml |
| With Pressure Cap | 15 ml |
| Dimensions | |
| Total Length | 116 mm |
| Width | 30 mm |
| Active Membrane Area | 6.0 cm² |
| Hold-up volume membrane | <20 µl |
| Dead stop volume | 50 µl |
| Carrier Required | |
| Insert Diameter | 29 mm |
| Conical Bottom Tube | 50 ml |
| Maximum Pressure | 5 bar |
| Materials of Construction | |
| Body and Filtrate Vessel | Polycarbonate |
| Concentrator Cap | Polypropylene |
| Membranes | Polyethersulfone |
| Pressure Head | Acetal & Aluminum |
| Centrifuge | ||
| Rotor Type | Swing bucket | Fixed angle |
| Minimum rotor angle | - | 25° |
| Rotor Cavity | To fit 50 ml (30 mm) conical bottom tubes | To fit 50 ml (30 mm) conical bottom tubes |
| Maximum Speed | 4,000 g✝ | 6,000 g✝ |
| Concentrate Recovery | ||
| Pipette type | Fixed or variable volume | Fixed or variable volume |
| Recommended tip | Thin gel loader type | Thin gel loader type |
✝ Please note, devices with membrane MWCO ≥ 100 kDa need to be processed at lower g forces. See data sheet for details.
| Description | Prod. # | Price | Buy |
|---|---|---|---|
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3,000 MWCO, PES Membrane, 12 per pack
SKU: VS2091
Price: $188.00 |
Add to Cart | ||
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3,000 MWCO, PES Membrane, 48 per pack
SKU: VS2092
Price: $683.00 |
Add to Cart | ||
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5,000 MWCO, PES Membrane, 12 per pack
SKU: VS2011
Price: $188.00 |
Add to Cart | ||
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5,000 MWCO, PES Membrane, 48 per pack
SKU: VS2012
Price: $683.00 |
Add to Cart | ||
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10,000 MWCO, PES Membrane, 12 per pack
SKU: VS2001
Price: $188.00 |
Add to Cart | ||
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10,000 MWCO, PES Membrane, 48 per pack
SKU: VS2002
Price: $683.00 |
Add to Cart | ||
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30,000 MWCO, PES Membrane, 12 per pack
SKU: VS2021
Price: $188.00 |
Add to Cart | ||
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30,000 MWCO, PES Membrane, 48 per pack
SKU: VS2022
Price: $683.00 |
Add to Cart | ||
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50,000 MWCO, PES Membrane, 12 per pack
SKU: VS2031
Price: $188.00 |
Add to Cart | ||
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50,000 MWCO, PES Membrane, 48 per pack
SKU: VS2032
Price: $683.00 |
Add to Cart | ||
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100,000 MWCO, PES Membrane, 12 per pack
SKU: VS2041
Price: $188.00 |
Add to Cart | ||
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100,000 MWCO, PES Membrane, 48 per pack
SKU: VS2042
Price: $683.00 |
Add to Cart | ||
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300,000 MWCO, PES Membrane, 12 per pack
SKU: VS2051
Price: $188.00 |
Add to Cart | ||
|
300,000 MWCO, PES Membrane, 48 per pack
SKU: VS2052
Price: $683.00 |
Add to Cart | ||
|
1,000,000 MWCO, PES Membrane, 12 per pack
SKU: VS2061
Price: $188.00 |
Add to Cart | ||
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1,000,000 MWCO, PES Membrane, 48 per pack
SKU: VS2062
Price: $683.00 |
Add to Cart | ||
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0.2µm, PES Membrane, 48 per pack
SKU: VS2072
Price: $683.00 |
Add to Cart |
| Description | Prod. # | Price | Buy |
|---|---|---|---|
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Air Pressure Controller (APC)
SKU: VCA002
Price: $465.00 |
Add to Cart | ||
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Charge valve for pressure head
SKU: VCA005
Price: $87.50 |
Add to Cart | ||
|
Female connector
SKU: VCA010
Price: $70.90 |
Add to Cart | ||
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Male connector
SKU: VCA011
Price: $58.70 |
Add to Cart | ||
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4 mm OD pneumatic tube (3 ml)
SKU: VCA012
Price: $13.20 |
Add to Cart | ||
|
Vivaspin 20 pressure head
SKU: VCA200
Price: $85.30 |
Add to Cart | ||
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Diafiltration cups, 12 per pack
SKU: VSA005
Price: $34.40 |
Add to Cart |
Up to 500 µl starting volume
Concentrate up to 100x
Up to 2 ml starting volume
Concentrate up to 250x
Up to 4 ml starting volume
Concentrate up to 200x
Up to 4 ml starting volume
Concentrate up to 100x
Up to 6 ml starting volume
Concentrate up to 200x
Up to 15 ml starting volume
Concentrate up to 150x
Up to 15 ml starting volume
Concentrate up to 400x
Up to 100 ml starting volume
Option of Pressure-fugation or Gas pressure filtration in addition to centrifugation
Up to 2.5 ml starting volume
For very viscous or particle-containing liquids
