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Vivaspin® Filtrate Concentrator

Up to 2.5 ml starting volume
Concentrate up to 40x

Vivaspin Filtrate centrifugal devices allow concentration of difficult to filter samples, such as whole blood or cell lysates, up to 2.5 ml in volume. They can be used in rotors accepting 15 ml centrifuge tubes. The unique piston design allows ultrafiltration to take place in the direction opposite to the centrifugal force and prevent premature blocking of the membrane. The filtrate is collected in the inserted piston tube which is easily withdrawn to then pipette the concentrate.

Data Sheet

Technical Data and Operating Instructions

Concentration of fungal antibodies prior to complement fixation or immunodiffusion for detection of Coccidioides and Aspergillus.
Concentrate bacterial antigens (H. influenzae, S. pneumoniae) prior to latex particle agglutination for detection of sepsis in newborns.
Protein removal from serum & cell / tissue lysates.
Prepare samples for mass spectrometry.

	Time to filter 50% of sample volume	Time to filter 90% of sample volume	Passage of sample species volume
BSA 1.0 mg / ml (66,000 MW)
5,000 MWCO CTA	300 min	N / A	0%
10,000 MWCO CTA	35 min	80 min	2%
20,000 MWCO CTA	9 min	20 min	2%
IgG 0.25 mg / ml (160,000 MW)
100,000 MWCO PES	13 min	35 min	3%
Blue Dextran 0.1 mg / ml (2,000,000 MW)
300,000 MWCO PES	9 min	25 min	28%

* 2.5 ml samples were loaded into each device.
The devices were centrifuged at 2,000 g until the required filtrate volumes had been reached.

Concentrator Capacity
Swing Bucket Rotor	2.5 ml
Fixed Angle Rotor	2.5 ml
Dimensions
Total Length	93 mm
Width	14 mm
Active Membrane Area	0.79 cm²
Hold-up volume of membrane	< 5 µl
Dead stop volume	100 µl
Materials of Construction
Centrifuge tube	Polystyrene
Floater tube	Styrene acrylonitrile
Cap	Polyethylene
Membrane	CTA, PES

Centrifuge
Rotor Type	Swing bucket	Fixed Angle
Minimum rotor angle	-	25°
Rotor Cavity	To fit 15 ml (17 mm) conical bottom tubes	To fit 15 ml (17 mm) conical bottom tubes
Maximum Speed	2,500 g	2,000 g
Concentrate Recovery
Pipette type	Fixed or variable volume	Fixed or variable volume
Recommended tip	Thin gel loader type	Thin gel loader type

Description	Prod. #	Price	Buy
5,000 MWCO, Cellulose Triacetate Membrane, 12 per pack SKU: 13229-E Price: $99.10	13229-E	$99.10	Add to Cart
10,000 MWCO, Cellulose Triacetate Membrane, 12 per pack SKU: 13239-E Price: $99.10	13239-E	$99.10	Add to Cart
20,000 MWCO, Cellulose Triacetate Membrane, 12 per pack SKU: 13249-E Price: $99.10	13249-E	$99.10	Add to Cart
100,000 MWCO, Polyethersulfone Membrane, 12 per pack SKU: 13269-E Price: $99.10	13269-E	$99.10	Add to Cart
300,000 MWCO, Polyethersulfone Membrane, 12 per pack SKU: 13279-E Price: $99.10	13279-E	$99.10	Add to Cart