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Rapid separation of free drugs and hormones
Vivafree™ 500 centrifugal filters offer fast, accurate separations of free drugs for Therapeutic Drug Monitoring. They have been used successfully for phenytoin1, valproic acid, antibiotics, immunosuppressants and other drugs. Vivafree™ filters are also being used clinically to separate free hormones such as testosterone and insulin. Separations can be performed as quickly as 20 minutes with high recoveries and excellent reproducibility. Savings of 35% have been reported when switching to Vivafree™ filters from another centrifugal ultrafiltration device2. Vivafree™ 500 filters are intended for in vitro diagnostic (IVD) applications and are designed for use in fixed angle rotors.
(1) Comparison of Centrifugal Filter Performance for Free Phenytoin Assays (PDF)
(2) Evaluation of a Centrifugal Filtration Device in the Measurement of Free Testosterone (PDF)
| Device Type | MWCO | Test Sample (all from serum) |
Initial Volume |
Filtrate Volume |
Time | Temp. | Centrifugal Force (x g) |
| Vivafree™ 500 | 125,000 | Free Insulin | 500 µl | 150 µl | 30 min. | 37° C | 2,000 |
| Vivafree™ 500 | 30,000 | Free Piperacillin | 500 µl | 100 µl | 30 min. | 25° C | 2,500 |
| Vivafree™ 500 | 30,000 | Free Tazobactam | 500 µl | 100 µl | 30 min. | 25° C | 2,500 |
| Vivafree™ 500 | 30,000 | Free Linezolid | 500 µl | 90 µl | 20 min | 37° C | 1,000 |
| Vivafree™ 500 | 30,000 | Free Fluconazole | 500 µl | 90 µl | 20 min. | 37° C | 1,000 |
| Vivafree™ 2 | 30,000 | Free Cortisol | 500 µl | 100 µl | 20 min. | 37° C | 2,500 |
| Vivafree™ 2 | 30,000 | Free Phenytoin | 1,000 µl | 200 µl | 20 min. | 25° C | 1,500 |
| Vivafree™ 2 | 30,000 | Free Testosterone | 300 µl | 100 µl | 20 min. | 37° C | 4,500 |
| Vivafree™ 2 | 30,000 | Free Testosterone✝ | 1000 µl✝ | 500 µl | 60 min. | 25° C | 4,500 |
✝ This sample starts with 1000 µl of diluted serum. Free Testosterone analyzed by mass spectrometry.
Note: Filtration times may vary according to sample volume and quality. Lipemic samples may require longer spin times. Filtration rates in swinging bucket rotors are typically 2-3 times slower compared to fixed angle rotors.
| Concentrator Capacity | |
| Swing Bucket Rotor | 0.5 ml |
| Fixed Angle Rotor | 0.5 ml |
| Dimensions | |
| Total Length | 45 mm |
| Width | 12.4 mm |
| Active Membrane Area | 0.32 cm² |
| Hold-up volume, membrane and support | <5 µl |
| Dead stop volume (fixed angle rotor*) | 5 µl (40° rotor) |
| Dead stop volume (swing bucket rotor*) | No dead stop |
| Materials of Construction | |
| Body | Polycarbonate |
| Filtrate Vessel | Polypropylene |
| Back Spin Vial | Polypropylene |
| O Ring | Silicone |
| Membrane (ex 125,000 MWCO) | Hydrosart® |
| Membrane (125,000 MWCO) | Cellulose Triacetate |
| Equipment Required - Centrifuge | |
| Rotor type | Fixed Angle or Swinging Bucket ✝ |
| Rotor Cavity | To fit 1.5 ml | 2.2 ml (11 mm) conical bottom tubes |
| Maximum speed 30,000 MWCO |
5.000x g |
| Maximum speed 125,000 MWCO |
2,500x g |
✝ Filtration rates in swinging bucket rotors are typically 2-3 times slower compared to fixed angle rotors.
| Description | Prod. # | Price | Buy |
|---|---|---|---|
|
30,000 MWCO, Hydrosart Membrane, 25 per pack
SKU: VFR01H21
Price: $135.00 |
Add to Cart | ||
|
30,000 MWCO, Hydrosart Membrane, 100 per pack
SKU: VFR01H22
Price: $430.00 |
Add to Cart | ||
|
30,000 MWCO, Hydrosart Membrane, 500 per pack
SKU: VFR01H23
Price: $1,850.00 |
Add to Cart | ||
|
125,000 MWCO, Cellulose Triacetate Membrane, 25 per pack
SKU: VFR01H81
Price: $135.00 |
Add to Cart | ||
|
125,000 MWCO, Cellulose Triacetate Membrane, 100 per pack
SKU: VFR01H82
Price: $430.00 |
Add to Cart | ||
|
125,000 MWCO, Cellulose Triacetate Membrane, 500 per pack
SKU: VFR01H83
Price: $1,850.00 |
Add to Cart |
