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Vivafree™ 500 Centrifugal Filter

Up to 500 µl starting volume
Rapid separation of free drugs and hormones

Vivafree™ 500 centrifugal filters offer fast, accurate separations of free drugs for Therapeutic Drug Monitoring. They have been used successfully for phenytoin¹, valproic acid, antibiotics, immunosuppressants and other drugs. Vivafree™ filters are also being used clinically to separate free hormones such as testosterone and insulin. Separations can be performed as quickly as 20 minutes with high recoveries and excellent reproducibility. Savings of 35% have been reported when switching to Vivafree™ filters from another centrifugal ultrafiltration device². Vivafree™ 500 filters are intended for in vitro diagnostic (IVD) applications and are designed for use in fixed angle rotors.

(1) Comparison of Centrifugal Filter Performance for Free Phenytoin Assays (PDF)

(2) Evaluation of a Centrifugal Filtration Device in the Measurement of Free Testosterone (PDF)

Device Type	MWCO	Test Sample (all from serum)	Initial Volume	Filtrate Volume	Time	Temp.	Centrifugal Force (x g)
Vivafree™ 500	125,000	Free Insulin	500 µl	150 µl	30 min.	37° C	2,000
Vivafree™ 500	30,000	Free Piperacillin	500 µl	100 µl	30 min.	25° C	2,500
Vivafree™ 500	30,000	Free Tazobactam	500 µl	100 µl	30 min.	25° C	2,500
Vivafree™ 500	30,000	Free Linezolid	500 µl	90 µl	20 min	37° C	1,000
Vivafree™ 500	30,000	Free Fluconazole	500 µl	90 µl	20 min.	37° C	1,000
Vivafree™ 2	30,000	Free Cortisol	500 µl	100 µl	20 min.	37° C	2,500
Vivafree™ 2	30,000	Free Phenytoin	1,000 µl	200 µl	20 min.	25° C	1,500
Vivafree™ 2	30,000	Free Testosterone	300 µl	100 µl	20 min.	37° C	4,500
Vivafree™ 2	30,000	Free Testosterone✝	1000 µl✝	500 µl	60 min.	25° C	4,500

✝ This sample starts with 1000 µl of diluted serum. Free Testosterone analyzed by mass spectrometry.

Note: Filtration times may vary according to sample volume and quality. Lipemic samples may require longer spin times. Filtration rates in swinging bucket rotors are typically 2-3 times slower compared to fixed angle rotors.

Concentrator Capacity
Swing Bucket Rotor	0.5 ml
Fixed Angle Rotor	0.5 ml
Dimensions
Total Length	45 mm
Width	12.4 mm
Active Membrane Area	0.32 cm²
Hold-up volume, membrane and support	<5 µl
Dead stop volume (fixed angle rotor*)	5 µl (40° rotor)
Dead stop volume (swing bucket rotor*)	No dead stop
Materials of Construction
Body	Polycarbonate
Filtrate Vessel	Polypropylene
Back Spin Vial	Polypropylene
O Ring	Silicone
Membrane (ex 125,000 MWCO)	Hydrosart®
Membrane (125,000 MWCO)	Cellulose Triacetate
Equipment Required - Centrifuge
Rotor type	Fixed Angle or Swinging Bucket ^✝
Rotor Cavity	To fit 1.5 ml \| 2.2 ml (11 mm) conical bottom tubes
Maximum speed 30,000 MWCO	5.000x g
Maximum speed 125,000 MWCO	2,500x g

^✝ Filtration rates in swinging bucket rotors are typically 2-3 times slower compared to fixed angle rotors.

Description	Prod. #	Price	Buy
30,000 MWCO, Hydrosart Membrane, 25 per pack SKU: VFR01H21 Price: $140.00	VFR01H21	$140.00	Add to Cart
30,000 MWCO, Hydrosart Membrane, 100 per pack SKU: VFR01H22 Price: $440.00	VFR01H22	$440.00	Add to Cart
30,000 MWCO, Hydrosart Membrane, 500 per pack SKU: VFR01H23 Price: $1,900.00	VFR01H23	$1,900.00	Add to Cart
125,000 MWCO, Cellulose Triacetate Membrane, 25 per pack SKU: VFR01H81 Price: $140.00	VFR01H81	$140.00	Add to Cart
125,000 MWCO, Cellulose Triacetate Membrane, 100 per pack SKU: VFR01H82 Price: $440.00	VFR01H82	$440.00	Add to Cart
125,000 MWCO, Cellulose Triacetate Membrane, 500 per pack SKU: VFR01H83 Price: $1,900.00	VFR01H83	$1,900.00	Add to Cart