Proteus NoEndo

Proteus NoEndo™

Proteus NoEndo™ spin columns provide fast convenient affinity-based removal of endotoxins from antibodies and other proteins. These devices combine quality separations found in gravity columns with the speed of centrifugal separators. Using the unique FlowGo™ design, about 99% of endotoxins can be removed in a single pass through the agarose resin with protein recoveries of 85% or greater. High Capacity (HC) columns are available for recombinant proteins expressed from bacteria such as E. coli and Standard (S) columns are offered for purification of monoclonal antibodies from mammalian cells. NoEndo™ resin is also available separately for larger sample sizes. The Micro kit is also available for small sample sizes.

Performance Characteristics

  Micro Standard High Capacity
Typical in situ binding capacity 300-500 EU 30,000 EU 500,000-1,000,000 EU
Typical binding capacity per ml resin 500-800 EU/ml 1,500 EU/ml 30,000 EU/ml
Minimum endotoxin levels tested post-column <0.03 EU/ml <0.05 EU/ml <0.05 EU/ml
Typical endotoxin clearance after 1 pass   3 log reduction 3 log reduction
Typical endotoxin clearance after 2 passes   4 log reduction 4 log reduction
Typical endotoxin clearance after 1 hour incubation 3 log reduction    
Typical endotoxin clearance after 3 hour incubation 4 log reduction    
Maximum sample load volume 0.6 ml 20 ml 20 ml
Bed volume 0.01-0.1 ml loose 1 ml pre-packed 1.7 ml pre-packed
Bead size range 100 µm 100 µm 100 µm
Recommended working pH 4-8 4-8 4-8
Color coded end-caps Supplied in vials Light green Dark green

Technical Specifications

  NoEndo™ S & HC NoEndo™ Micro
Sample Capacity (by rotor type)
Swinging bucket 20 ml NOT USABLE
Fixed angle 12 ml (25°) 0.6 ml
Dimensions
Length 118 mm 48 mm
Width 29 mm 11 mm
Hold-up volume 10 µl 2-3 µl
Materials of Construction
Outer tube Polypropylene Polypropylene
Inner tube Polyethylene Polypropylene
Resin sintered plug Polyethylene N/A
NoEndo™ resin Cross-linked 6% agarose Cross-linked 6% agarose
Centrifuge Required
Rotor cavity To fit 50 ml conical bottom tubes To fit 2.2 ml centrifuge tubes
Maximum Speed 1,500 x g
(Fixed angle or Swinging Bucket)
13,000 x g
(Fixed angle ONLY)

Proteus NoEndo™ Ordering Information

Description Prod. No. Price
2 spin columns, 2 centrifuge tubes, 2 micro resin cartridges, 1 insertion tool GEN-NoE2MICRO $67.00
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2 spin columns, 2 centrifuge tubes, 2 standard resin cartridges, 1 insertion tool GEN-NoE2S $115.00
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2 spin columns, 2 centrifuge tubes, 2 high capacity resin cartridges, 1 insertion tool GEN-NoE2HC $135.00
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12 spin columns, 12 centrifuge tubes, 12 standard resin cartridges, 1 insertion tool GEN-NoE12S $460.00
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12 spin columns, 12 centrifuge tubes, 12 high capacity resin cartridges, 1 insertion tool GEN-NoE12HC $540.00
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24 spin columns, 24 centrifuge tubes, 24 micro resin cartridges, 1 insertion tool GEN-NoE24MICRO $300.00
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48 spin columns, 48 centrifuge tubes, 48 standard resin cartridges, 1 insertion tool GEN-NoE48S $1,540.00
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48 spin columns, 48 centrifuge tubes, 48 high capacity resin cartridges, 1 insertion tool GEN-NoE48HC $1,800.00
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100 spin columns, 100 centrifuge tubes, 100 micro resin cartridges, 1 insertion tool GEN-NoE100MICRO $990.00
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100ml NoEndo loose resin suspended in 20% ethanol GEN-NoE0100ML $1,312.50
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